Olive is a holly, evergreen, subtropical and long life tree that is native to Mediterranean area. The usual method of propagation of this tree is semi-hardwood cutting. The cuttings of olive are more difficult to root. Rooting disability can be efficient in developing of new orchards with appropriate, adaptable and acclimated cultivars. In this research, the effect of two growth regulators indol -3- butyric acid (IBA), putrescine and their interaction on the root regeneration and subsequent growth of semi-hardwood cuttings of olive were studied. In the first experiment which has performed in 1389, three concentrations of IBA (0, 2000 and 4000 mgl -1 ) and three concentrations of putrescine (0, 0.1 and 1 Mm) were used on ، Zard , and ، Roghani , cultivars. The experiment was carried out as 3×3×2 factorial experiment in a completely randomized design whit four replications. Results showed that the percentage of healthy and rooted cuttings were higher in ، Zard , cultivar, compared with ، Roghani , . Treatment of cuttings with 2000 mgl -1 IBA increased total percentage of cutting with callus production and root regeneration, root numbers and root length per cutting, branching and number of branches per cutting compared with control and 4000 mgl -1 IBA Putrescine at the concentrations of 0.1 and 1 mM showed the best results regarding percentage of cuttings with callus and root production, numbers and length of root per cutting and new shoot produced on cuttings. A significant interaction between IBA and putrescine was observed so that the largest percentage of healthy and rooted cuttings were obtained in combination of 2000 mgl -1 IBA with 0.1 and 1 mM putrescine. In the second experiment which was done in 1390, only ، Zard , cultivar was used. Treatments were combination of 2000 mgl -1 IBA with one of the three putrescine concentrations of 0, 0.1 and 1 mM. The experiment was done as a completely randomized design with four replications. Sample of cuttings were taken for measurments in 3 times, two, three and four months after cutting. In this study two levels of 0.1 and 1 mM putrescine increased percentage of cuttings with callus and root production, numbers and length of root per cutting, branching and number of shoots per cutting compared to control treatment, whereas there was no significant differences between these two concentrations. Also by reviewing the effect of IBA and putrescine along time, results revealed that the percentage of cuttings with callus were higher after two months from cutting and treating with IBA and putrescine compared with three and four months, whereas rooted cuttings were higher at three and four months sampeling. By the time, the number and length of the roots and also number of shoots per cutting were increased. Overall, use of 0.1 and 1 mM putrescine following treatment of cutting with 2000 mgl -1 IBA were better treatment, so that it can help rooting and following growth of the cuttings for both olive cultivars, so it can be used for improving propagation method of this plant. Key words : Olive, indol -3- butyric acid, putrescine, cutting, callus and rooting.