The aim of this study was to investigate the effects of two kind of inoculants, ECOSYL (containing Lactobacillus plantarum) and LALSIL (containing lactobacillus plantarum and propionibacterium acidipropionici) and addition of 0, 5 and 10 percent molasses (dry matter basis) on nutritive value and fermentation quality of wilted and unwilted alfalfa (Medicago Sativa) forage in laboratory scale. Alfalfa forage in forth cut harvested at the prebud stage of maturity with 20 percent of dry matter. It was divided to two parts, part 1 was chopped directly to a theoretical length of cut of 25 mm with a forage chopper and part 2 was chopped after field wilting to approximately 37 percent of dry matter. Each part was separately divided to three parts and each of these parts was combined with 0, 5 and 10 percent of molasses on dry matter basis. Finally each part was divided to three parts as part 1 was inoculated by LALSIL, part 2; ECOSYL and part 3; the same quantity of distilled water. All treatments were ensiled in 3.5-L mini silos equipped with a lid enabling gas release only. Results indicated that wilting forage prior to ensiling increased dry matter and limited fermentation, increased residual water soluble carbohydrate but decreased organic acid production and proteolysis as well. Wilting forage did not affect Lactic acid to acetic acid ratio and digestibility. Totally, despite of increase in pH, silage quality was better in wilted silages than unwilted ones. Adding molasses decreased silage pH and acidic condition was led to decreased proteolysis. Adding molasses increase acetic acid content of silage and this compound was led to higher dry matter losses in fermentation phase and decreased nutritive value of silage. Adding high level of molasses increased water soluble carbohydrate and In-vitro dry matter digestibility. Inoculants induced homolactic fermentation, increased efficiency of lactic acid production and residual water soluble carbohydrate. Rapid decline in pH decreased acetic acid production and proteolysis. Inoculants increased in-situ dry matter digestibility. Interaction effect of molasses and inoculants showed that bacterial inoculants with high levels of molasses, increased water soluble carbohydrate and digestibility and decreased acetic acid production. Interaction effect between wilting and molasses indicated that in unwilted silage, addition of molasses declined lactic acid production but in wilted silage addition of molasses did not affect lactic acid production. Also both levels of molasses decrease ammonia N in unwilted alfalfa, but in wilted silage only high level of molasses showed this reduction. Bacterial inoculants had better response in wilted alfalfa than unwilted. In wilted silage both of the inoculants decrease pH and proteolysis. Result of our experiment indicated that bacterial inoculants and molasses supplementation when used simultaneously, improved sil