Water deficit is an important environmental stress which adversely affects crop production in many agricultural areas. The effects of water deficit can be reduced by inoculation of plant roots with mycorrhiza fungi or by application of super absorbent materials in soil. This experiment was conducted to evaluate the effects of the inoculation of plants with mycorrhiza and the use of super absorbent on the morphological and physiological characteristics of berseem clover at different levels of soil moisture. For this purpose, the inoculation of plants with five species of mycorrhiza including: Glomus etunicatum , G. versiforme , G. mosseae , G. intraradice s, and G. fasciculatum were tested in the first experiment. In the second experiment, the inoculation with Glomus intraradice s and the application of super absorbent were evaluated on the growth characteristics of berseem clover under three irrigation regimes (100, 50, 30 % of field capacity). There were significant reductions in plant height, leaf area, shoot and root dry weight, total and special root surface, root length, the concentration of P in the roots, leaf protein percentage, and the mycorrhizal colonization of plant roots at lower compared to higher levels of soil moisture. In contrast, the root diameter and the leaf proline concentration were significantly increased under water stress. Plant height, leaf area, shoot and root dry weight, shoot/ root ratio, the concentration of P, Zn and Cu in shoot and roots, total and special surface of roots, and root length were significantly increased in inoculated compared to uninoculated plants. In general, compared to other tested species, Glomus intraradice s was superior in terms of colonization and its positive effects on growth and nutrient uptake of the plants. There were significant interactions between irrigation regimes and the application of mycorrhiza on shoot dry weight, leaf area, root surface, and root length. The extent of the increases in plant dry weight of inoculated plants was greater at lower compared to higher levels of soil moisture. The application of super absorbent in soil caused significant increases in plant height, leaf area, shoot and root dry weight, shoot/ root ratio, total and special surface of root, and root length. The results from this study showed that the inoculation of plants with mycorrhiza can improve the performance of berseem clover under water stress through its positive effects on nutrient uptake of the plants. Moreover, the application of super absorbent materials can mitigate the adverse effects of water deficit on the growth of berseem clover in low watering conditions. Keywords: Berseem clover, Water deficit, Mycorrhiza, Super absorbent, Growth parameters, Nutrient uptake.