Water deficit is one of the major limiting factor affecting growth and yield of plant and the issue of world limate change make it more . One of the way to increase tolerance against the water stre i plant i inoculatio with plant- growth promoting rhizobacteria .The use of these microorganisms as bio- fertilizer induce the increase in plant growth and development, improvement nutritional statu plants important in tress full condition. The gladiolu flower ( Gladiolus . (L.) Dalenii) is a plant from Iridaceae family which is cultivated for cut flower and ornamental aspect . The present experiment was conducted as a factorial experiment based on CRD to study gladiolus flower response to inoculation with two species plant-growth promoting rhizobacteria ( Pseudomonas fluorescence , P. putida) of and two irrigation levels (75 and 100 %FC) with three replications. The results showed that the number of florets, height and chlorophyll florescence index decreased significantly under water stress. The greenne of leaf reduced under water deficit conditio but inoculatio with P. flourescenc at full irrigation conditio resulted in improved greenness of leaf. Also the result showed that chlorophyll a content, total chlorophyll and carotenoids decrease at 75%FC however inoculation whit P. flourescenc was significantly improved them . By reducing the irrigation water, proline content was increased at 75%FC and inoculation by P. putida at 75% FC increased 14.84% it compared with them. Also with increasing water stress malondialdehyd content was increased significantly at75% FC and inoculation with P. fluorescence , P. putida in full irrigation caused 45.16% reduction compared to water deficit condition . The results showed that the activity of guiacol peroxidas and superoxide dismutase wa significantly increased in water deficit and inoculation with P. fluorescence caused a reduction in the activity of guiacol peroxidas. Also activity of superoxide dismutase whit P. putida was significantly increased by 71 %. To sum u the result show that the positive effects of inoculation with gladiolus plant resulted in improve chlorophyll content, improved enzymatic and non enzymatic defense mechanism resulted i increased tolerance in stress full and non stress full conditions and it can be useful i destructive effects of water deficit and it seems providing sufficient irrigation is necessary for gladiolus production. Keywords : Water deficit, Plant- Growth Promoting Rhizobacteria, Gladiolus, Chlorophyll, Defense mechanism, Stress