Soil salinity is an important factor limiting agricultural production. Numerous physiological and morphological processes of plant are affected by soil salinity mainly via the osmotic potential of the soil solution and uptake and accumulation of mineral ions. Canola as a semi salt-tolerant oilseed crop is considered to be an amenable plant for doubled-haploid system due to high embryo induction and plant regeneration from microspore culture. The main objectives of the present study were to evaluate the effects of salinity stress on physiological traits using 95 double haploid lines and 5 spring cultivars which grown in the field of College of Agriculture, Isfahan University of Technology located in Lavark, Najafabad with two simple latice design (10×10) under salinity sress and non-stress conditions. The following traits were evaluated: leaf concentrations of sodium, potassium, calcium, carbohydrate, proline, aldehyde malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), potassium/sodium ratio, calcium /sodium ratio. Combined analysis of variance showed the significant effect of salinity on most traits in way that the salinity increased the concentrations of sodium, calcium and carbohydrates (82.35, 32.46 and 50.41%. respectively), while the concentrations of potassium, potassium/sodium ratio, MDA and H 2 O 2 decreased (53.84, 37.06, 24.34and 6.81 %, respectively). The results of the estimation of heritability for the traits showed that the highest and lowest heritability estimates belonged to proline and potassium content under non-stress as well as potassium and MDA content under salinity stress conditions, respectively. Genetic and phenotypic correlation coefficients between sodium with potassium (negative) and potassium/sodium ratio (positive) as well as between MDA and H 2 O 2 (positive) were significant. Factor analysis explained four factors that can justify over 70% of total variation of the traits. The cluster analysis dendrogram based on the physiological traits divided the 100 genotypes into three 0in 0in 0pt" dir=rtl Keywords: Canola, doubled haploid, salinity stress, factor analysis
