The edible mushroom Agaricus bisporus is the most abundant industrially cultivated mushroom, which constitutes an important component of the food basket of people in the world because of its appropriate nutritional quality, especially high content of protein. On the other hand, we know that the use of vitamins, hormones, and amino acids improves the vegetative growth of the mushroom and improves its overall production in different flashes of harvest. In this sense, this study was conducted in two separate experiments in order to evaluate the effects of vitamins B and C, IBA hormone and amino acid tryptophan on the growth and yield of the button mushroom in the mushroom cultivation house and its postharvest life during the storage. A completely randomized block design with factorial arrangements in three replications was employed using three levels of vitamin C (0, 0.5, and 1 mg/kg), three levels of vitamin B (0, 3, and 6 mg/kg), three levels of IBA (0, 0.001, and 0.002 M/kg), and three levels of tryptophan (0, 3150, and 6300 mg/kg). The results showed the effectiveness of feeding supplements on the vegetative growth of the mushroom, with in the first flash the greatest number of mushroom and the highest dry weight were obtained with vitamin B treatment and the highest content of vitamin C, harvest weight, ash percentage, fresh weight, base diameter, and percentage of ionic leakage were obtained with vitamin B treatment, while the tryptophan treatment had the greatest yield of second- Keywords: Button mushroom, tryptophan, vitamin C, vitamin B, IBA, whiteness