Pulsed electric field (PEF) destructs biological cells and cell membranes rupture and it is used to extract fruit and vegetable juice and remove water. In addition to, high voltage electric fields deactivate microbial activities. Background researches show that ultrasound waves causing cavitation while and microwave causing heat penetration into the plant tissue in order to damage molecule cells. In the this study, three treatments applied to study effects of pulse electric field, microwave and ultrasound on the efficiency of essential oil of thyme ( Thymus vulgaris L.) with using to Clevenger procedure, total phenolic content using Folin Ciocalteu method and antioxidant activity by the method of trapping DPPH radical. Moreover, these variables were measured for Methanol extraction of thyme. The treatments were PEF intensities of 1, 1.5, and 2 kV per centimeter, with 500, 100, and 1000 microseconds pulse width, and pulse train at three levels of 20, 60, and 100 pulses; ultrasound wave with the power of 40 W and a frequency of 30 kHz at three ranges of 20, 40, and 60 minutes; microwave with 200 watts power and a frequency of 4.2 GHz at three levels of 1, 3 and 5 minutes. The treatments were compared in a complete randomized design. The effect of PEF on the cell destruction thyme index showed that with enhancement the electric field intensity and the number of pulses, the destruction of the cells increased. With increases at intensity of the PEF, efficiency of thyme essential oil was increased, but the number of pulses and pulse width had no significant effect. Ultrasonic waves and microwaves had no effects on essential oils extraction due to heat and its evaporation. Microwave thyme extraction significantly increased with total phenolic content compared to the control. No significant difference was observed between the antioxidant activities of essential oils, but all three treatments enhanced the antioxidant activity compared to the control. Combination of the two treatments for the antioxidant activity of samples had no efficiency. Combination of the PEF and ultrasound wave, and combination of microwave and PEF had significantly difference with respect to either treatment alone in the total phenolic content extraction. Keywords: pulsed electric field, ultrasonic, microwave, essential oil, extract, thyme