The goal of this study is to increase the production of lipids and lutein in Scenedesmus obliquus microalgae, biogas production from its biomass residues and pretreatment to increase biogas production. In order to produce lutein, different laboratory cultures were performed with different shocks. Nitrate concentration increased from 0.25 to 3 g / L and light intensity was increased from 25 to 130 ?mol-ft / m 2 . s. It was observed that in this condition the lutein content decreased compared to other studies. The highest lutein content was obtained in two-stage culture. In the first stage we used light intensity of 25 ?mol-ft / m 2 . s nitrate and nitrate concentration of 0.25 g / L for ten days and in the second stage, nitrate concentration of 3 g / L and light intensity of 130 ?mol-ft / m 2 . s were used for 4 days. As a result, lutein content of 0.087 mg / g biomass, protein content of 62% and higher concentration of biomass (1.33 g / L) were obtained compared to the control sample (0.45 g / L). In a one-stage culture that used a light intensity of 130 ?mol-ft / m 2 . s and a nitrate concentration of 3 g / L, the lutein content was 0.064 mg / g biomass and 68% protein was obtained. Furthermore, biomass concentration increased significantly (2.2 g / L). In this study, in order to achieve high biomass content with high lipid content in Scenedesmus obliquus microalgae, a two-stage method based on complete culture medium in the first stage and then culture under restricted conditions (transfer of sedimented biomass to stage Second) and nitrogen deficiency (transfer of centrifuged biomass to the second stage) was evaluated. The results showed that two-stage photoatotrophic culture for 9 days in the first stage with nitrate concentration of 0.25 g / L and 5 days of nitrate shock in the second stage, lipid content of 9% for nitrate restriction and deficiency to 12 and 18.3, respectively. The percentage of biomass weight and in mixotrophic culture with the same conditions increased from 8 to 11 and 13% of biomass weight. Mixotrophic cultures increased from 8 to 11 d 13% biomass weight, respectively. Photoatotrophic cultures with nitrate deficiency were selected to increase lipid content. Different pretreatments such as ultrasonic, thermal and hydrothermal were used to break the cell wall of microalgae and increase the efficiency of methane production. In most cases, methane production efficiencies increased. Since the C / N ratio of this algae species (8.5-9) is lower than the optimal ratio (20-30 C / N) for the anaerobic digestion process, rice straw is used as a digestion aid to increase the C / N ratio substrate was used. moreover, in most cases, satisfactory results were obtained compared to the literature. Lipid extraction residue from biomass was used by ultrasonic method to produce biogas and 192.48 ml / g of volatile material was obtained. By adding digestion aid (rice straw) in a ratio of 1: 3 (algae: straw), the methane production efficiency of this sample increased to 606.6 ml / g of volatile matter. As a result, Scenedesmus obliquus microalgae are suitable species for increasing lipid content and biodiesel production, they are not suitable species for increasing lutein content with bold basel culture medium, and high methane can be produced by breaking the cell wall of this algae species. key words: cenedesmus obliquus , lutein, protein, lipid, chlorophyll, biofuel, biodiesel, biogas