In this project, colloidal gas aphrons is used for purification of glucoamylase from solid state fermentation media. Three different experimental methods were used as the effective methods for recovery of glucoamylase. Also, the cationic and anionic surfactant were tested for a pH range. The volume of aphrom to enzyme solution, pH and concentration of flocculation agent were investigated. Good results were obtained for combination method that activity recovery and purification factor were about 100% and 2, respectively.
