Turfgrasses are the most common plants in landscapes. Bermudagrass ( Cynodon dactylon L. Pers.) has a great importance because of it ’s resistance to drought, salinity and traffics. Iran is one of the centers of diversity and development of this plant. Therefore subtle evaluation of these rich resources, regarding morphological traits and determination of genetical diversity among them, for doing breeding activities and causing more qualified cultivars would be useful. This study evaluated genetical diversity among 23 bermudagrass and four hybrid cultivars (Tifdwarf, Tifgreen, Tifway, Midlawn) using morphological traits and ISSR molecular markers and then, the best of accessions were selected for breeding activities. Fourtheen quantitative morphological traits and 20 qualitative morphological traits were respectively plant height, rhizome diameter, flag leaf length and width, internode gap, length and number of spikelets, number of nodes below main inflorescence axis, length of hair in flag leaf ’s collar region, length of upper glume and down glume, length of lemma and palea, width of flag leaf ’s ligule and fleshy rhizomes, hairy node, hairy stem in collar region, softness of leaf, inflorescence colour, spikelet state, hairy florettail, similarity of upper and down glume, shape of upper and down glume, hairy upper and down glume margine, shape of palea and lemma, hairy palea and lemma margine, statement of lemma’s ending, flag colour and days to flowering were measured. After all, 14 primers were used for ISSR experiments. Cluster analysis divided genotypes to three group basing on quantitative morphological traits and to four groups basing on qualitative morphological traits and also to four groups basin on ISSR molecular markers. It was considered that in first dendrogram (which was based on quantitative traits), four hybrid cultivars were belonged to the same group with 4/75 Euclidean distance and these four hybrid cultivars belonged to the same group in second dendrogram with 4/75 Rogers distance as well. Furthermore, existing of two Iranian diploid accessions (17 and 88) in same group with 5 Euclidean distance in the first dendrogram and with 9 Rogers distance in the second dendrogram, enforced the probability of being derived from common basement. In cluster analysis of ISSR moleqular markers, Midlawn hybrid cultivar, which are cold hardy compared with Tif series, was separated from hybrid cultivars of Tif series. Accessions of each group located in this dedrogram had similarity ploidy levels. Results of this analysis to main phenomenons of ISSR molecular markers showed that these markers are dispersed on genome level. Investigation o