The pulses, including cheetah bean have a great contribution to human nutrition by having a significant protein content. Moreover, drought is one of the most important environmental stresses that negatively affects plant growth and development stages and consequently grain yield. The present study aimed to investigate the effect of different irrigation levels on the growth and as well identification of some physiological indicators of stress tolerance in cheetah bean genotypes. The experiment was conducted at National Bean Research Station, in Khomein, using a 3-replicate split plot RCBD in 2019. The irrigation regimes as main plot consisted 50 (I 1 ), 70 (I 2 ) and 110 (I 3 ) of cumulative evaporation using atandard cla"A"evaporation pan.18 cheetah bean genotypes were considered as subplot. Drought stress reduced the relative water content, chlorophyll a , chlorophyll b , plant height, 100 seeds weights, the number of seeds per pod, the number of pods per plant and the number of seeds per plant. The rate of this decrease was higher with increasing water stress intensity. However, the proline and malondialdehyde content increased due to water stress. The amount of grain protein significantly increased by 5 and 11%, respectively, at I 2 and I 3 compared to I 1 irrigation level with a decrease in using irrigation water. Under control irrigation regime (I 1 ), a negative and significant correlation was observed between grain yield and protein content and positive and significant correlation between grain yield with grain number in pod and proline content. Besides, under severe water stress (I 3 ), the correlation between grain yield and number of pods per plant, number of seeds per pod and number of seeds per plant was positive and significant. The difference between the studied cheetah bean genotypes was significant (p?0.01) in terms of tolerance and sensitivity indexes to mild drought stress (I 2 ). Among the studied genotypes, the highest and lowest mild drought tolerance index belonged to Ghaffar (1.17) and KS-21486 (0.387) genotype and the highest and lowest mild drought sensitivity index observed in KS-21573 (1.81) and KS-920054 (0.207) genotype, respectively. While there was significant differences (p?0.05) related to severe stress (I 3 ) tolerance index between studied genotypes. The highest and lowest severe drought stress tolerances belonged to Ghaffar (1.12) and KS-21486 (0.373) genotype, respectively. Due to the grain yield of the studied genotypes, Ghaffar cultivar is introduced for normal irrigation conditions and KS-21318 genotype for mild and severe drought stress conditions in the study area. In general, it can be stated that there was a significant diversity among the studied genotypes in response to different levels of irrigation that can be used to improve and select cheetah bean for drought tolerance. Key words : Drought, Grain yield, Food Safety, Product Stability, Tolerant genotype