The fast economical growth in the last century, Caused increasing consumption of Fossil fuels. Pollutants of Fossil Fuel combustion, environmental hazards, limited resources and its inadequacy to supply energy in the near future, has created a requirement to replace fossil fuels with renewable and refined fuels. One of the best and cheapest sources to replace fossil fuels is the energy from biofuels. Different Types of waste materials, such as organic wastes from livestock industries that are abundantly found or derived from the production or consumption of human food, can be used as the sources for the production of biofuels such as biogas. By using biologilcal processes and extraction of energy from organic wastes in the form of biogas, the productivity of this materials can be increased. In this research, it has been tried to investigate some factors affecting the of biogas production efficiency. In general two types of physical and chemical pretreatment including ultrasonic, macrowave, heating to 106 °C, addition of iron oxide , copper and aluminum nano particle to cow manure and egg powder was done. In order to provide environmental conditions for biogas producing microorganisms, reactors of 300 ml volume containing substrate and inoculum were considered as batch and placed in an incubator at 37 ° C for 45 days. In order to measure the volume of produced biogas, the reactors were connected to the column of water by biogas transfer pipes. A total of three 45-day period experiments were carried out and the results showed a positive effect for heating pretreatment and a negative effect for sonicate pretreatment. The addition of iron oxide, copper and aluminum nanoparticles resulted in a 21.4% increase at best case, 22.9% and 22.9% decrease at worst case, respectively. The reactor which contained egg powder substrate produced 6.1% biogas more than the reactor which contained cow manure. The result of the study of volatile fatty acid at the end of the reactions indicated the presence of nutrients for the production of more biogas by reactors containing egg powder substrate. The Analysis of Produced biogas showed the positive effect of nanoparticles on the percentage of methane in the biogas composition. Analysis of the composition of cow manure before and after the biogas production processes showed that the C / N ratio has been changed from 26 to 13.26, 15.12 and 14.69 for reactors with different predecessors corresponding to the volume of biogas. Keywords: Biogas, Cow manure, Egg powder, Nano iron oxide, Nano copper, Nano aluminum oxide