The purpose of the current thesis is to build, evaluate and optimize an energy generator based on glucose fuel cell (GFC) capable of operating in conditions near to the physiological conditions of the human body, in the following three studies: In the first part of the thesis, a non-platinum catalyst based on Fe-Co /C with an average particle size of 3 nm is introduced as the cathode of the GFC. Its activity and performance has been evaluated using a three-electrode assembly and a GFC, respectively and the results has been compared with a conventional Pt/C catalyst. The results show that the Fe-Co/C cathode shows the oxygen reduction reaction (ORR) peak in phosphate-buffered saline () and also selectively reduces oxygen in the presence of glucose, amino acids and also in a real human blood serum. During 11 hours under a discharge current of 0.2 mA cm-2 in containing glucose, the cathode potential drop was 16%, indicating that the cathode selectivity performed ORR in the presence of glucose. In the second part of the thesis, studies covered the anode side of the GFC including the introduction and evaluation of Pt/rGO (reduced graphene oxide) for glucose oxidation in an abiotic GFC in medium. During 15 hours of constant current discharge of 0.01 mA cm-2, a 23% drop in cell voltage occurred while the fuel ( containing 5 mM glucose) was stationary. When the fuel was circulated in anode side (based on human arm’s vein blood flow rate, 0.33 mLs-1), the cell voltage increased by 10% due to the improvement of glucose mass transfer on the surface of the anode electrode. In the third part, the performance of an abiotic GFC (anode: Pt/rGO and cathode: Fe-Co/C) as an energy generator is evaluated and compared with an abiotic GFC (anode: commercial Pt/C) in three physiological environments: /glucose, /glucose/amino acids, and real human blood serum. The cell voltage drop during 8 hours under constant load of 0.01 mA cm-2 in /glucose /amino acids and serum media were 9.87 and 17.25 mV h-1, respectively which are much higher than the ideal medium (/glucose) which was 1.5 mV h-1. In addition, in real human blood serum, the efficiency of the GFC utilizing the synthetized Pt/rGO anode catalyst is higher than that of a GFC utilizing commercial Pt/C anode catalyst which were 12.5 µW cm-2 (under 54 µA cm-2 discharge load, 230 mV voltage) compared to 4.6 µW cm-2 (under 45 µA cm-2 discharge load, 103 mV voltage)
