In this study nanofibers were prepared via the electrospinning technique using basil seed mucilage and its application for encapsulation of hespertin was investigated. Basil seed mucilage and PVA solution with concentration of 2% and 10% were used, respectively. Viscosity and electrical conductivity of BSM/PVA solutions under different mass volume ratios (100: 0, 80:20, 60:40, 40:60, 20:80 and 0:100) were survied. Morphology of nanofibers was investigated using scanning electron microscopy (SEM) that showed an average diameter range of 179-390 nm. Nanofibers produced with volume ratio of 60:40 and fabricated under voltage of 18 kV were selected as the best fiber and used for future analysis by Fourier transform infrared spectrometer (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and encapsulation. Hesperetin were added in three levels (10%, 15% and 20%). The FTIR spectrum of BSM/PVA/Hesperetin nanofibers showed that no chemical reaction was occurred between BSM, PVA and hesperetin. X-ray diffractograms indicated that the crystalline index of BSM, PVA, BSM/PVA nanofibers, hesperetin and BSM/PVA/hesperetin nanofibers were 54, 35, 43, 40 and 56%, respectively. An increase in thermal stability of hesperetin after encapsulation was observed. Encapsulation efficiency (EE%), loading capacity (LC%) of 20% hesperetin nanofibers were 88.74± 17.1065 and 65.13± 0.12, respectively. The results showed that release profile in 2 first hours in gastric media, next 6 hours in the intestine media and for whole release time (8 hours) were 15.87%, 35.1 and 50.97%, respectively. To assess the mechanism of hesperetin release from fibers, release fractions as a function of time were analyzed by second order, Higuchi, Horsmeyer-Peppas, Hixson-Crowell and Weibull models. Based on statistical coefficients for each model in gastric and intestine media, the release kinetics for hesperetin from BSM /PVA/hesperetin fibers showed the best correlation with the Korsmever Peppas model with R 2 of 0.9998 and 0.953, respectively. Keywords : Basil seed mucilage; Electrospinning; Hesperetin; Kinetic, Rrelease