In the first section of this study, a new sensor is introduced for determination of flutamide based on reduced graphene oxide (rGO) and ferrite nickle nanoparticles (NiFe 2 O 4 ) for the modification of glassy carbon electrode. The surface morphology, structure, properties and electroanalytical behavior of NiFe 2 O 4 -rGO were studied using transmission electron microscopy (TEM), fourior transform infrared spectroscopy (FT-IR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Flutamide was successfully determined with linear range of 0.24-40.00 µmol/L and detection limit of 0.18 µmol/L. Finally, the proposed electrochemical sensor was successfully applied for determination of flutamide in urine and blood serum samples. In the second section of this study, the synthesis and use of novel and cheep nanomaterials cobalt iron layered double hydroxide (Co/Fe LDH) decorated on graphene as an electrocatalyst to produce hydrogen and oxygen in alkaline solution were studied. The surface morphology, structure, properties and electrocatalyst behavior of the electrocatalyst was studied using transmission electron microscopy (TEM), fourior transform infrared spectroscopy (FT-IR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and linear sweep voltammetry (LSV). In this work, a modified glassy carbon electrode (GCE) with (Co/Fe LDH) decorated on graphene was used. The results of our studied showed that Co/Fe@G-LDH exhibits high electrocatalytic activity and long term stability for oxygen and hydrogen evolution raections.