In this project, an electrochemical sensor based on Ni 3 S 2 nanoparticles supported on ball-milled porous silicon flour was fabricated for measuring glucose. At first, the glassy carbon electrode surface was modified by Ni 3 S 2 nanoparticles supported on ball-milled porous silicon flour substrate. In order to characterization the modified electrode, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectroscopy (EDX), Elemental mapping and X-ray diffraction (XRD) were used. In the following, the effective parameters on the sensor response such as pH, NaOH concentration, amount of the electrocatalyst, applied potential and rotational speed of the electrode were optimized using cyclic voltammetric and hydrodynamic amprometric methods. Under the optimal conditions, calibration curve was drawn using hydrodynamic amprometric method. Three linear regions were obtained from 0.5-134, 134-1246, and 1246- 3546 µmol L -1 glucose, with a detection limit of 0.2 ?M. Finally, the proposed method was used for determination of glucose in human blood plasma samples.