Carnivores such as Caracal ( Caracal caracal ) are located in the top of food pyramid and play important roles in ecosystems. Different factors such as deficiency of prey and habitat destruction have reduced the species population. One of the key parts in management and conservation of such predators is to understand what they eat, which means what preys’ type and size they exactly feed on. Food habits of large carnivores are associated with important ecological features such as spatial pattern, movement, habitat selection, social structure, geographical distribution and reproductive success. Caracal is an average sized predator belonged to the felid family, lives in arid and semi-arid areas and few studies have been conducted on this species. In the present study, Caracal’s diet in Abbas Abad Wildlife Refuge was investigated using fecal analyses. In this regard, 22 fecal samples were collected from the animal dens during four visits in the region. Faeces were washed on sieve and the remaining components were identified using morphological and DNA extraction methods. Results showed a diversity in food items consumed by Caracals in Abbas Abad Wildlife Refuge. 65.38% of the diet was related to rodents such as Gerbillus nanus , Meriones persicus , Allactaga elater and Mus musculus , which formed the maximum portion, 7.69% was related to birds, 3.84% antelope ( Capra aegagrus) and 7.69% insects. In this study, residuals of livestock were not found within Caracal food items. The survival of each predator is directly dependent on the food diet’s quantity and quality, therefore, recognition of Caracal’s food resources and conservation of prey populations might be a solution for better management and to improve the conservation status of this species.