A novel molecularly imprinted electrochemical sensor for sensitive determination of carbamazepine was developed. nano porous gold electrode was used for enhancement of sensitivity. The thin film of molecularly imprinted sol-gel polymers with specific binding sites for carbamazepine was cast on nano porous goldelectrode by electrochemical deposition method. The resulting composite was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetric techniques (CV). The interaction between the imprinted sensor and target molecule was observed by measuring the current response of the oxidation–reduction of the [Fe(CN) 6 ] 3- /[Fe(CN) 6 ] 4? as a probe, on the electrode. Under the optimal experimental conditions, the response peak current was linear to the concentration of carbamazepine in the range from 2 to100 nM and 100 to 1000 nM the detection limit was 0.7 nM and RSD was 4%. This imprinted sensor was successfully applied to the determination of carbamazepine in human and tablet samples. These results have revealed that the imprinted sensor fulfilled the selectivity, sensitivity,speed and simplicity requirements for carbamazepine detection and provided possibilities of clinical application in physiological fluids.