Bioactivity and antibacterial properties are important factors properties in tissue engineering. So, in this project, Chitosan-Tragacanth (CS-TG) film was fabricated under ultrasonic irradiation via casting solution with having bioactivity properties. After that, titanium(IV) oxide (TiO2) nanoparticles () fillers and TiO2-Ag hybrids were applied for increasing these properties. The amounts of 2, 5, and 8 % weights of TiO2 respect to a total weight of CS-TG polymer were used in this film. Next, field emission scanning electron microscopy images of films showed that TiO2 of CS-TG/TiO2 nanocomposite (NC) film 5% was the best dispersed than CS-TG/TiO2 NC films 2 and 8 %. After that, CS-TG/TiO2 NC film 5% was selected, and TiO2-Ag hybrid was added with the same amount of this, but the hybrid deferent in related in two . In this project, Silver (Ag) were synthesized green chemical methods, and these doped to TiO2 via ultrasonic irradiation. A related amounts of TiO2:Ag were doped 1.00:0.50, 1.00:0.75, and 1.00:1.00. The image of transmission electron microscopy shows that TiO2-Ag hybrid was better dispersed than TiO2 in the CS-TG blend polymer. Finally, the bioactivity of CS-TG/TiO2 and CS-TG/TiO2-Ag NC films were assayed. The result of bioactivity shows that all of the films have bioactivity properties. In addition, this test shows that various shapes of Hydroxyapatite (HA) were prepared on the surface of NC films. Probably, the various shape of HA were related the amount of Ag to TiO2 in the CS-TG film. As well as, the antibacterial properties of NC films were studied. CS-TG/TiO2 and CS-TG/TiO2-Ag NC films were examined with a well-diffusion method via two kinds of bacterias Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative). Also, all of the samples were studied under exposure to ultraviolet (UV) irritation and dark condition. CS-TG/TiO2-Ag 1,00:1,00 NC film related Ag to TiO2 shows better antibacterial activity than other samples under exposure to UV irradiation and dark.
