Microalgae due to their easier harvesting and processing, faster growth, high carbon stabilization efficiency, less need to water and soil, and the ability to use waste for growth, have been introduced as desirable sources of lipids (for biodiesel production), carbohydrates (as feed for fermentation processes such as bioethanol production), and valuable products such as proteins, carotenoids and bioactive compounds; But dependence on light, culture medium preparation, and downstream processes, including cell degradation, extraction, and purification, are bottlenecks in the large-scale production of microalgae and their products. The aim of this study was to investigate the conditions for growth and content of carotenoids and carbohydrates (for bioethanol) for the development of the green microalga Chlamydomonas starrii biorefinery. The study of the effect of light on this microalga showed that a moderate light intensity of about 2000 Lux with a biomass yield of 5.9 mg. L????. day???? is the most suitable light intensity for the growth of C.starrii. Sodium bicarbonate concentration of 2 g/L for 6 days gives the highest biomass productivity (59.2 mg. L????. day????) in anaerobic photo-autotrophic culture. Different stress conditions including nitrogen and phosphorus deficiency, high concentrations of NaCl and nitrate, and high light intensity did not increase the carotenoid content of C.starrii cells in two-stage culture (10 days of growth and 10 days of stress condition), indicating that the major carotenoids of its cells are primary (such as lutein, zeaxanthin, and ?-carotene). Phosphorus stress causes the greatest decrease in carotenoid content and increase in chlorophyll-b content. Among the mentioned stress conditions, nitrogen stress, despite the experience of notable carbohydrate reduction in the first days of shock, shows the highest cellular carbohydrate content (76.2%) on the last day of the stress period. Glucose concentration of 1 g/L for 5 days gives the highest biomass productivity (103.8 mg. L????. day????) in anaerobic photo-heterotrophic culture. Glycerol concentration of 7 g/L during 7 days gives the highest biomass production efficiency (60.7 mg. L????. day????) in anaerobic photo-heterotrophic culture. The maximum biomass concentration in glycerol (0.364 g/L) is not less than glucose (0.323 g/L) but the growth period in glycerol is longer. Keywords: Microalgae, Chlamydomonas, Stress, Carotenoid, Carbohydrate, Bioethanol.