In this project, optical sensors have been designed based on carbon quantum dots (CQDs) and these sensors have been used to determination Doxorubicin. The CQDs are synthesized by using a hydrothermal method and using Tragacanth gum as a carbon source. Different methods such as UV–Vis, TEM, DLS,EDX, Fourier transform infrared (FTIR) and Raman spectroscopy have been used to confirm the synthesis of CQDs. The variable factors of optical sensor response for doxorubicin, including pH, the amount of carbon dots and the time of the reaction, were optimized. In optimal conditions, the optical sensor response for doxorubicin in the linear range ane detection limit were calculated 1.0-400.0 g mL -1 calculated 0.4 ng mL -1 respectively . The relative standard deviations for the two concentrations of 10.0 and 200.0 of doxorubicin with 5 repeats were calculated 2.1% and 1.4%, respectively. The advantages of this optical sensor are high sensitivity, comfortable synthesis, selectivity and acceptable accuracy. Eventually, this sensor was used to determination doxorubicin in real samples. Subsequently, a new, inexpensive and simple optical sensor was developed using the placement of CQDs on a Boehmite mesoporous (AlOOH) surface based on hydrogen bonding. The surface-to-volume ratio, the presence of high-volume pores, increases the longevity and sustainability of CQDs; also prevents nanoparticles from stacking. The variable factors influencing of optical sensor response for determination of were evaluated and optimized. In optimal conditions, the linear range was calculated from 1.0 to 500.0 ng mL -1 and the detection limit of 0.2 ng mL -1 was used. The relative standard deviation percentage for both concentrations of 20.0 and 150.0 ng mL -1 of Doxorubicin with 5 repetitions was 1.3% and 0.9%, respectively. The sensor designed for measuring doxorubicin in human blood plasma was used.