Colour is an important part of our daily life which can influence our feelings. Colour information such as hue, lightness and chroma has the potential to affect our perceptions, emotional reactions and behavioral reactions. A single colour or a combination of colours can be associated with a certain feeling. Knowing about the emotional effect of colours can be useful in product design and marketing, and it is defined as the relationship between colour stimuli and the reactive-level of emotional response, which is determined by configuration of colour stimuli in a visual experience. Colour emotion is a multidisciplinary research area which is involved in colour science, psychology and etc. The aim of the present research is to clarify the relationship between colour and emotion and to investigate the effects of culture, gender and age on colour emotion. To achieve this aim, psychophysical experiments were carried out. Forty observers took part in the experiments to evaluate the colour stimuli using “heavy - light”, “warm - cool”, “active - passive” and “like - dislike” colour emotional scales. These scales were chosen from a wide range of suggested emotional scales in former studies. The observers divided into three different groups to investigate the effect of culture, gender and age on different colour emotional scales. The observer groups were female versus male, from Isfahan versus not from Isfahan and 20 to 30 years old versus 35 to 45 years old. Twenty colour stimuli which covered CIELAB colour space reasonably, were presented on a calibrated LCD in a darkened room. The observers were asked to report their emotional responses to each of the stimuli. The reported data were transformed to scale values using categorical judgment method. The results showed that the effect of gender, culture and age on the “heavy - light”, “warm - cool” and “active - passive” colour emotional scales is insignificant, but these factors have significant effect on “like - dislike” response of observers. Also the relationship between the CIE colour appearance attributes, L*, a* and b*, and the colour emotional scales were investigated. It was found that the “heavy - light” colour emotional scale is in connection with L* through a negative linear relationship. The “warm - cool” colour emotional scale is in association with chroma and hue angle as the “warm” stimuli are located in red-orange-yellow region of CIELAB colour space and the “cool” ones are located in green-purple-blue region of CIELAB colour space. Finally, the “active - passive” colour emotional scale has a relationship with chroma. Examining the suggested models of former studies showed a good correlation between visual data and predicted ones. Findings of present research can be useful in a designing process and enable designers to have the best colour choices through a wide range of possible selections in interior design, textile design and etc.