Carbon nano tubes are a proper candidate for reinforcing fibers, thanks to their remarkable mechanical, chemical, electrical and thermo-mechanical properties. Fabricating of composites reinforced with carbon nano fibers and nanotubes show both improved physical and mechanical properties and evaluating instinct properties of nano fibers. In present research, first an appropriate dispersion of nylon 66 solution and functionalized carbon nanotubes (different percentages) has been produced. To functionalize the as received CNT, in other words constructing carboxyl group on them, the CNT was created in a mixture of H 2 SO 4 and HNO 3 (3:1) and sonication. In the second stage the dispersion was electrospun and nylon()66 nanofiber s containing CNT produced. Then, effects of carbon nanotubes on mechanical, structural and electrical properties of nanofibers were assessed. Stress-strain test demonstrated that an optimal concentration of 15 percent would result in 127 percent increase in Young’s module of the nano-web as well as 139 percent increase in strength of nano-web. DSC results showed that crystallinity of nanofibers with 15 percent carbon nanotube increased to 46.3 percent from 33.67 percent for nylon 66 nanofibers. In addition, the test showed that cold crystallization temperature increased to 244.27 °Cfrom 232.54°C. XRD results showed a good correlation between DSC results. An increasing trend in the rate of crystallinity of nano fibers with increased amount of carbon nanotube has been demonstrated. Only the 20 Percent CNT specimen showed lower mechanical properties and crystallinity when compared with15 percent CNT specimen. This is because of the accumulation of carbon nanotubes. Increasing CNT content in the electrospun ano-webs led to an increase electrical conductivity as well as a lower Discharging time.