In the present study, in order to achieve an inexpensive tolerable anode catalyst for direct methanol fuel cell applications, a composite of polyaniline (PANI) Nano-fibers and Pt/C Nano-particles, indicated by PANI/Pt/C, was prepared by in-situ electropolymerization of aniline and trifluoromethane sulfonic acid on the glassy carbon. The effect of synthesized PANI nanofibers in methanol electrooxidation reaction was compared by bare Pt/C by different electrochemical methods such as; cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry. Scanning electron microscopy (SEM) was also employed to observe the morphology of the modified catalyst layer. The test results reveal that introduction of PANI Nanofibers within catalyst layer improve the catalyst activity in methanol oxidation, hinder and prevent catalyst from more poisoning by intermediate products of methanol oxidation and improve the mechanical properties of the catalyst layer.SEM images are also indicate that Porous PANI Nano-fibers make good electrical juctions between platinum particles and anchor platinum particles and alleviate the Pt migration during methanol electrooxidation.