Zinc de?ciency is one of the most widespread micronutrient de?ciencies in plants that disorder in the production of wheat and other cereal crops. An increase in reactive oxygen species (ROS) levels and a decrease in efficiency of detoxi?cation mechanisms may be the major reasons for impairment of various cellular functions in Zn-de?cient plants. Given that vast areas of land under wheat cultivation in arid and semi-arid parts are zinc deficient, Hence the increase plants potency to tolerance this element efficiency is important in terms of yield losses. Biological methods based on soil useful microorganisms’ potentials in creating symbiosis relationship to plants can increase crops yields per unit area by changing their genetic structure and improving their cultivation in zinc deficient soils. This study was conducted in order to investigate the in?uence of individual and simultaneous inoculation of endophytic fungus, Piriformospora indica and plant growth-promoting rhizobacterium, Pseudomonas putida and Azotobacter chroococcum on physiological and biochemical properties of two wheat cultivars nicknejad and azadi in response to zinc deficiency stress. The experiment was conducted in greenhouse soilless culture center of Isfahan University of technology by using the sterile sand-perlite (2:1 v/v), in a completely randomized design with three replications. The results showed that nicknejad cultivar than with azadi cultivar had the less zinc efficiency and tolerance to zinc deficiency stress. Highest shoot dry weight at zinc efficiency and sufficiency conditions was belonged to individual inoculation of A. chroococcum and P. putida , respectively, however the impact these bacteria in zinc inefficient nicknejad cultivar was more significant. Individual inoculation of P. indica and A. chroococcum and P. putida bacteria at zinc efficiency condition resulted in increase the total amount of zinc in shoot of nicknejad cultivar but their impact on the total amount of zinc in shoot of azadi cultivar was not significant. Zinc deficiency resulted in increase iron concentration in shoot of nicknejad cultivar and phosphorous concentration in shoot of both cultivars. Highest phosphorous and iron concentrations in shoot of wheat cultivars belonged to individual inoculation of P. indica . But, individual inoculation of A. chroococcum and P. utida reduced phosphorous concentration in shoot of wheat cultivars at both levels of zinc compared to non-inoculation of bacteria. Also at zinc efficiency condition, individual inoculation of A. chroococcum and P. putida reduced iron concentration in shoot of nicknejad cultivar compared to non-inoculation of bacteria. At zinc efficiency condition, the highest chlorophyll a and b and carotenoids concentrations of azadi cultivar was achieved by individual inoculation of P. putida that with individual inoculation of P. indica and simultaneous inoculation of P. indica and P. putida in a group were statistixly. However, the highest chlorophyll a and b and carotenoids concentrations of nicknejad cultivar at zinc efficiency condition belonged to individual inoculation of P. indica and simultaneous inoculation of P. indica and P. putida. Individual inoculation of A. chroococcum at zinc efficiency and sufficiency conditions, resulted in reducing chlorophyll a, b and carotenoids concentrations of nicknejad cultivar. Individual inoculation of A. chroococcum and P. putida played an important role in inducing antioxidant enzyme activities of ascorbate peroxidase and peroxidase of wheat cultivars in response to zinc deficiency stress. However, individual inoculation of P. indica only in increase enzyme activitie of ascorbate peroxidase of azadi cultivar and enzymes activity of peroxidase of nicknejad cultivar was successful. Enzyme activity of Catalase of nicknejad cultivar at zinc efficiency condition, was not affected by microbial inoculation. In general, inoculation of wheat plant with plant growth-promoting rhizobacterium can serve as a useful method for alleviating deleterious effects of zinc deficiency stress in sensitive cultivar to this element efficiency. Keywords : Zinc deficiency, Wheat, Pseudomonas putida ، Azotobacter chroococcum and Piriformospora indica .