The growing population of the earth has led to an increased demand for food resources. Since, livestock and poultry have always been important food sources for mankind, this population growth has enhanced the activity of the slaughterhouse industry and consequently, increased waste from these activities. Not using these animal waste, despite causing environmental pollution, can play an important role in raising the final price of poultry and meat; Thus, the conversion of these waste materials into value-added, seems inevitable. In the study described here, these wastes were alkali pretreated after characterization to replace peptone as an integral part of the culture medium. For this purpose, Design-Expert software was used to design the experiments for determining the optimum hydrolysis point of the feather meal. According to the results, the optimum hydrolysis occurs at 76.5 °C, ratio 8, time 180 min and 0.5 M KOH solution concentration. Subsequently, the livestock
