Magnetic susceptibility approach as a simple, relatively inexpensive, fast, and non-destructive method has been used in a wide range of rocks and soils studies in the past few decades. Although the measurement of magnetic susceptibility is simple but interpretation of results is complicated and typically requires knowledge of the effective factors controlling it. This study aimed to examine the vertical distribution of magnetic susceptibility, to determine the changes in magnetic susceptibility after removing some of the effective factors, and to estimate minerogenic magnetic susceptibility in soils path along the Isfahan-Chelgerd transect having an arid to semi-arid climate. Major soil great groups along the transect including 7 pedons in the Isfahan region with an aridic soil moisture regime and a thermic temperature regime and 8 pedons in the ChaharMahalVaBakhtiari region with a xeric moisture regime and a mesictemperature regime were selected and sampled to the depth of 150 centimeters. Samples were taken for laboratory analysis from70 genetic horizons belongingto 15 profiles.To determine the effect of diamagnetic materials on magnetic susceptibility, desalination and degypsification treatments were carried out on soils from Isfahan using successive washing with distilled water and organic matter was removed from soils of ChaharMahalVaBakhtiari using H 2 O 2 30%. Decalcification treatment using the buffer solution of acetic acid - sodium acetate with pH 5 and removal of iron treatment by citrate- dithionite- bicarbonate solution were performed on all the soils.The results showed that the organic matter usually added to the soil surface, as well as salinity, gypsum and carbonates due to their diamagnetic behavior and also because of their mobility in soil would influence the magnetic susceptibility. They normally reduce the amount of magnetic susceptibility through dilution. The effect of desalination treatment on the increase of magnetic susceptibility was not statistically significant indicating that the contribution of soil salinity to magnetic susceptibility was not significant due to the small mass of soils occupied by soluble minerals. Also, iron removal resulted in the instability andelimination of maghemite and, thus, reduced the magnetic susceptibility. Due to the lack of discontinuity, the vertical distribution of magnetic susceptibility in pedons fromChaharMahalVaBakhtiary is uniform. In contrast, soils from Isfahan showed great changes in magnetic susceptibility with depth which could be attributed to their non-uniform parent materials and anthropogenic influence. Unexpectedly, the amount of magnetic susceptibility after the removal of carbonates as a measure of soil development was higher in soils from Isfahan as compared to those fromChaharMahalvaBakhtiari.The removal of iron shows that the high magnetic susceptibility of soils in Isfahan is not the result of soil development but also it depends on differences in mineralogy of parent material in the studied landscapes.While a great portion of magnetic susceptibility in ChaharMahalvaBakhtiary soils seems to originate from pedogenically formed minerals such as maghemite.In future studies, investigators are encouraged to remove the effect of each controlling factor to more satisfactorily determine the real role of soil components on soil magnetic susceptibility to be used in soil development interpretation.To remove the effects of diamagnetic materials and to compare real changes, minerogenic basedmagnetic susceptibility should be calculated.