In recent year Alstroemeria has gained a lot of interest as a cut flower .Important reasons are diversity of color and flower shape, easy cultivation, low energy requirement and long vase life .In the other hand its value as bedding or pot plant is not covered to every one. Alstroemeria is propagated asexually trough rhizome division with low multiplication rate about five rhizome a year and contributes to the spread of viral diseases. For these reasons and heterozygosity of Alstroemeria plants use of micro propagation techniques for Alstroemeria propagation in higher amounts seems to be necessary. Direct organogenesis and plant propagation trough callus are desirable models for plant regeneration. In this study appropriate conditions were obtained for callus induction from halved ovary and direct organogenesis from immature inflorescent, hypocotyle and rhizome’s bud. For callus induction, halved ovaries were culture on both solidified and liquid MS medium contained various concentrations of 2, 4-D and NAA in combination with 0.5 mgl -1 BA. Best callus regeneration was done on liquid MS medium supplemented with 1.5 mgl -1 2, 4-D and 0.5 mgl -1 BA. For plant regeneration from this newly produced callus, different concentrations of BA were evaluated but plants were produced in none of the medium. For direct organogenesis, different parts of Alstroemeria plant were cultured on MS medium contained various concentrations of BA and KIN in combination with 1 mgl -1 NAA. In immature inflorescent higher shoot induction rate (2.24 shoots per explants)was observed in medium supplemented with 2.5 mgl -1 BA and 3.5 mgl -1 KIN and highest rooting percent was done in medium supplemented with 1.5 mgl -1 BA and 2.5 mgl -1 KIN. Addition of NAA had no effect on rooting of these explants and rooting was done in the same media which were used for shoot induction after 1-2 subculture regimes. In hypocotyle culture highest number of produced shoots was observed in medium contained 1 mgl -1 BA and 0.5 mgl -1 NAA and addition of BA had decreasing effect on multiplication rate from this parts. After transition of explants with shoots rooting media highest number of explants with root and longest produced roots were observed in media supplemented with 4 and 1 mgl -1 NAA in combination with 0.5mgl -1 BA respectively. In the case of rhizome’s bud highest number of induced explants was observed in media contained 4 mgl -1 BA and 0.5 mgl -1 NAA. Addition of BA caused decreasing of shoot length. Highest number of produced rhizome was done from culture of rhizome’s bud in media supplemented with 1mgl -1 BA. Karyotype analysis of Alstroemeria ligto hybrid showed that this specious is diploid and contained 16 chromosomes [2n=2x=16].karyotypic formula for haploid set was 6m+ sm
