Flotation Process is the most common process to produce copper concentrate from low grade copper ores. The process is based on the surface chemistry of valuable and gangue minerals which are modified by chemical reagents namely collectors, frothers and modifiers. The main goal of this research was to optimize the type and concentration of the reagents used in flotation plant of Bavanat Copper mine. The effects of various parameters such as the type and concentration of collector, pH modifier, depressant on grade and separation efficiency of the copper concentrate was investigated. Experiments were conducted in batch scale in a 2.5 L Denver cell. Results showed that Sodium Isopropyl Xanthate (SIPX) and Isopropyl ethyl thiocarbamate (IPETC) collectors have a better separation efficiency rather than Potassium Amyl Xanthate (PAX). In addition lime was a more suitable pH modifier rather than sodium carbonate and NaOH. A Response surface methodology (Center Composite Design) was used to evaluate the influence of SIPX concentration, IPETC concentration, pH and MIBC concentration on separation efficiency and concentrate grade. It was found that the maximum separation efficiency occurred at SIPX concentration 7 g/ton ore, IPETC concentration 8.56 g/ton ore, MIBC concentration 20 g/ton and pH 11. In this condition the separation efficiency of process was calculated to be 88%. Under the optimum conditions in the presence of CMC and A3-3 depressants, the grade of the rougher and cleaning (after two steps) tests were 3.4% and 22.7%.