Plant growth and productivity are affected by various abiotic stresses such as drought, high salinity and cold that decrease the water status of plant cells. plants respond and adapt to these stresses at physiological and biochemical levels. Molecular studies have showed that many genes with various functions are induced by stress conditions. These genes protect the cells against environmental stresses or regulate gene expression and signal transduction during stress conditions. It is now hypothesized that at least four independent regulatory systems (regulons) function in activation of stress-inducible genes under dehydration conditions. One of the important regulon is DREB (dehydration-responsive element-binding protein) transcription factor which is classified in to DREB1 and DREB2. The aim of the present study was to overexpress DREB1B in potato plants to investigate its effect on osmotic tolerance. A DNA cassette consisting of Arabidopsis DREB1B cDNA driven by CaMV35S promoter, was cloned into a pBI121 binary vector. The cassette was transferred into Agrobacterium tumefaciens LBA4404 and fallowed by transformation in to potato ( Solanum tuberosum ) using five different protocols. The presence and expression of the gene in transgenic plants were confirmed by PCR and RT-PCR techniques, respectively. Overexpression of DREB1B caused growth retardation and increased cold tolerance in transgenic plants compared with the control plants (studies on transgenic plants in drought and salt stress conditions are underway.). Increase tolerance and growth retardation in transgenic potato highlight the presence of genes responding to transcription factor DREB1B in this plant. These results suggest that the engineering of stress tolerant potato by incorporating regulon genes like DREB1B may be an efficient approach to minimize stress damage. Although the issue of growth retardation must be minimized using stress-inducible promoters before this is applicable.