Wool keratin is a protein with biodegradability and biocompatibility properties. These merits have attracted the attention of researchers. This natural polymer, in powder form, has found applications in hygiene, cosmetics, filtration, tissue engineering scaffolds and controlled drug release. In submicron size range and especially in nano dimensions, the properties and behavior of keratin like any other material changes drastically. Up to the present time, nanoparticles of polysaccharides, some synthetic polymers and proteins such as albumin, gelatin, fibroin, sericin and feather keratin have been produced. Among the processes capable of producing nanoparticles (emulsification, dissolution and electrospray), electrospray enjoys advantages such as production of smaller, more regular and spherical particles. In order to produce nanoparticles, the intended polymer must be dissolved in a or a number of suitable solvents. The solution is then fed to an electrospray set up. During electrospraying, the initial droplets formed at the exit of the nozzle, are continuously broken up as a result of the coulombic explosions until reaching the collector of the electrospraying set up. The size of the particles reaching the collector can be as low a few nanometers. This research aimed at producing wool keratin nanoparticle through electrospraying. First wool keratin was recovered from wool fibers by dissolving it in Mercaptoethanol. To prepare the electrospray solution, the recovered keratin was dissolved in formic acid. To find the proper solvent, a number of solvents were tried and formic acid was found to be the most suitable. Apart from the production of keratin nanoparticles, this research involved an investigation on the effect of important electrospray conditions such as polymer concentration, feed rate, voltage and nozzle-collector distance on the average particle size of the electrosprayed nanoparticles. As conditions of electrospray, a range of 15 – 25 kV, 10 – 20 cm, 0.02 – 0.04 ml/h was chosen for voltage, nozzle-collector distance, and feed rate, respectively. These ranges were chosen by trial and error so that proper keratin nanoparticles could be produces. The results showed that the proper concentration of keratin in formic acid for the electrospraying keratin nanoparticle was about 0.5 % (w/v). Higher concentrations led to production big particle sticking to each other. As far as electrospraying conditions are concerned, decreasing feed rate, increasing nozzle-collector distance and increasing voltage lead to lower average particle size. The smallest average keratin particle size produced in this research was 65 nm. The smallest particle size seen was 10 nm.