Urban landscape has dramatically changed due to development of urbanization and population growth. The recent related research has been greatly promoted by the easier availability of higher resolution remote sensing data and the development of Geogrophical Information System (GIS) and geospatial techniques, as well as other disciplines such as landscape ecology. Quantifying urban landscape is fundamental for monitoring and assessment of ecological and socioeconomic consequences of urbanization. The convergence of GIS, remote sensing and other related disciplines has helped in quantifying, monitoring, modeling and subsequently predicting the urban land use development. Isfahan city landscape has changed due to urban expansion and land use changes. The aim of this research was detection of spatiotemporal changes from 1955 to 2007. To achieve this aim, land use map of Isfahan was generated in 6 The comparison between transects showed that the gradient of land use changes and metrics in 1955 is more symmetric than 2007. The comparisons of metrics values showed that landscape pattern in city center have not changed significantly and the most changes occurred in the margins of transects. In general the results showed that mean patch size and standard deviation of patch size is higher in margins than in median blocks. Spatial pattern of urbanization could be quantified using landscape metrics with a gradient analysis approach. Key word : Landscape ecology, Gradient analysis, Mosaic analysis, Landscape metrics and Isfahan city