YellowTragacanth gum extracted from yellow Astragalus is one of the most important biological gums. Iran is one of major produceraround the world. Today, tragacanth gum is applicable in various foodprocessing industries, pharmaceutical and healthcare costs. Tragacanth gum is a biocompatible and biodegradable polysaccharide which adsorb lots ofattention due to its biological friendly characteristics. Given the newadvances made by scholars and global initiatives to support nanotechnology andnanomedicine, it seems that a revolution in modern medicine would be triggeredby the introduction of nanotechnology products.Nanobiotechnology is atechnology used in nanotechnology in biotechnology and is the most strategicpart of nanotechnology in medical sciences. Gene therapy uses non-viralcarriers to insert a gene into the cell with the aim of achieving a therapeuticeffect that, by transferring copies of the gene-related protein to the disease,provides feasibility for treatment with less complications.In this study wehave investigated the chemical structure of yellow Tragacanth . Tragacanth chemical structure contains a mixture of polysaccharides and minerals is verycomplex. The yellow Tragacanth gum sample was extracted in therangelands of the region (Batligeh Friedan) in the western region of Isfahanprovince. In order to determine mineral elements atomic absorptionspectrophotometer and HPLC method was used for the determination of sugars.calcium As well as sodium was in the maximum amount of regions. Infraredspectroscopy was used for characterization analysis. In the second part of thestudy, the synthesis of plasmid- containing nanocapsules and its applicationwas investigated.After making nanocapsules using calcium chloride sedimentationagent, SEM analysis was performed to evaluate the accuracy of the nanocapsule.The results confirmed the spherical structure of nanocapsules. in order tocreate the conditions for the simulation of the stomach and intestine,respectively, the hydrochloric acid solution was normal and the sodiumcarbonate solution was added to the dry nanoparticles.Cell experiments wereperformed to evaluate the loading and release rate of the gene byelectrophoresis of agarose gel. The results indicate the stability of thestructures in the simulated stomach conditions as well as the release of the plasmidfrom the ketone nanocapsules in the intestinal simulated conditions. Using theZetasizer and DLS, the particle size of 64.4 milliliters and the hydrodynamicdiameter of the particles were 364.4 nm. Keywords: Yellow Tragacanth, Drug delivery, nanocapsule,SEM, DLS.