Ferula ovina Boiss is related to Umbelliferae type. This kind of plants could be found in Mediterranean, Central Asia and Iran. This plant will grow up mostly in 400 to 700 mm rain with the average annual temperature 4.5 to 11.5 cg. on low depth to quite depth medium texture to heavy texture and neutral acidity to alkali. This plant is the most valuable fodder, medical protective plant which can be used in developing mountain range and also this plant is used in food and taste. This study was carried out with the aim of bioassay of cementitious nanoparticles in a coma and its antimicrobial properties in five rangeland sites including Tang-e-Dazad, Ghaleh-e-Ghouh, Gohardasht, Islamabad and Kharsank which are the most important Ferula ovina Boiss habitats in the west of Isfahan province and in the Fereidoun Shahr ciry were considered. The properties of silver nanoparticles were investigated using various methods such as transmitted electron microscopy, ultraviolet spectroscopy, electron microscopy, and infrared spectrometer transformation. X-ray diffraction was also used to confirm the crystalline structure of nanoparticles. In this study, the average size of silver nanoparticles was between 23.78 and 15.8 nm. In this research, the antimicrobial property of silver nanoparticles synthesized by the extract of Gram-positive bacteria (Staphy Lococcus Aureus and Bacillus Cereus) and Gram-negative bacteria (Escherichia Coli and Salmonella Typhimurium.) By disc diffusion, the Minimum Inhibitor Concentration (MIC) And the Minimum lethal concentration (MBC). The results of the disc diffusion methods indicated that the nanoparticles produced by the Ferula ovina Boiss plant had antimicrobial properties. The minimum concentration of silver nanoparticles that can grow microbial strains is 25 ?g / ml, the gram positive antimicrobials were obtained. Also, the amount of 35.6 ?g / ml, gram-negative antimicrobials was obtained. At low concentrations, these nanoparticles caused the death of Escherichia coli and Salmonella Typhimurium. In this study, it was determined that bacteria and their wall properties influence the resistance of gram-positive and gram-negative bacteria to nano particles of silver from the extract of the plant. It was also found that rangelands did not affect the antimicrobial properties of the formed nanoparticles. Keywords : Green synthesis, Antimicrobial activity, Ferula ovina Boiss, nanoparticles, silver