Organic materials by effect on the physical, chemical, biological and fertility properties of soil have been considered as one of the foundations parameters for soil productivity. In addition, due to the great importance of using these materials in arid and semi-arid soils, application of any composition which containing these substances can be useful. Investigation of activated nitrogen deposits gives useful information about evaluating the health, sustainability, land use management and application of fertilizer to minimize pollution resulting from nitrogen-containing compounds. Over 95% of the total soil nitrogen deposits have been included organic nitrogen which some part of them are in dissolved. Amino acids are kind of the SON resource which have low molecular weight. The main aim of this study includes determine the value of soluble organic N, net N mineralization, concentration total amino acids, and urease enzyme activity in some under treatment soils with organic fertilize by considering different wet condition. In this regard, two samples from Sharvdan and Khansar in Isfahan province have been collected. Then, treatment with different organic fertilize (10 gr carbon per one kg of soil), in two different of wet condition 0.3 and 0.5 water hold capacity (WHC) for 5 months in Incubation in 25 centigrade have been done and measured 4 times a months. Some analysis included analysis of the breakdown of societies (based on time and type of organic fertilizers), ANOVA and LSD mean comparison method were conducted based on completely randomized in a factorial design. The results of discriminant analysis showed that treatment with Chicken manure and also measurements of first months of treatment with of incubation relative to other incubation is separated as a distinct statistical community. Time trend of variation of net N mineralization, soluble organic N, and urease enzyme activity showed in first month of incubation these factors are maximum and then decreased and finally in the last month raised again but the concentration total amino acids in both samples presents increasing trend in first and reach the maximum in second months and then decreased during the incubation and finally in the last month raised again. The maximum value of net N mineralization, and urease enzyme activity was related to the Chicken manure treatment and the maximum concentration of soluble organic N was related to Fertilizer municipal waste compost. In treatment with 0.3 WHC, the value of amino acids was more while net mineralization and soluble organic of nitrogen and urease enzyme activity in 0.5 WHC showed more value. Results of ANOVA presented that the interaction between moisture, time and type of organic fertilizer for all parameters were meaningful at 0.001. Results of correlation between soluble organic N and total concentration amino acids indicate no significantly correlation while between net N mineralization, soluble organic N and total concentration of amino acids in combination of both soil and also for each soil there is a significant positive correlation. The results of this study presents Chicken manure has the maximum value of net N mineralization, total concentration of amino acids, and urease enzyme activity and also irst month incubation have maximum value of net N mineralization, total concentration amino acids, soluble organic N and urease enzyme activity. The maximum concentration of amino acids examined in 0.3 WHC while net N mineralization, soluble organic N and urease enzyme activity in 0.5 WHC presented maximum value. Keywords : soluble organic N, Net N mineralization, urease enzyme activity, total concentration amino acids, manure organic, discriminant Analysis.