In the present study, coupled Electrodialysis-Complexation technique for selective separation of chromium ions from aqueous solution containing copper ions was used. One laboratory size three compartment ED cell for studying the effect of five various factors such as Flow rate of feed, Voltage, the ratio of cation concentration, molar ratio of EDTA and pH of the feed on the removal of Chromium was applied. Because of decreasing the number of experiments and strong analysis of the data, Taguchi design of experimental method was used and the experiments were designed according to L16 Orthogonal array of Taguchi. The obtained data were used for studying the relative removal of total chromium to the removal of copper ions and the separation percentages of Cr(III) and Cr(VI). S/N and ANOVA analysis methods were used and the S/N curves showing the procedure off removal with changing the factors were studied and optimum conditions for achieving the maximum separation were got. Analyzing of the data showed the great potential of this method comparing to using ED alone. After arrangement of the experimental conditions according to the optimum circumstances specified with Taguchi method the maximum separation of 87% for trivalent chromium and 95% of hexavalent chromium and relative removal of chromium more than 3 were obtained. S/N and ANOVA analysis methods were used and the S/N curves showing the procedure off removal with changing the factors were studied and optimum conditions for achieving the maximum separation were got.