In this work, Stir bar sorptive extraction (E) and Atomic Absorption Spectrometry (AAS) and Atomic emission spectrometry with Ion Coupled Plasma (ICP) were used to preconcentration and measurement of Pb(II). Ion Imprinted Polymer (IIP) was also used to preconcentration and separation of Pb(II) in aqueous solutions. This IIP is the reaction product of Methacrylic acid (monomer), Ethylene glycol dimethyl acrylate (crosslinker) , 2,2- Azobis (2- methylpropionitrile) (initiator) and complex of Pb(II) (imprint ion) with 1,5 – Diphenylcarbazide in Ethanol. Before putting polymer, the glass bars have been placed in 3-Methacryloxypropyltrimethoxysilane and acetone solution for 1 hour to bond the polymer on the glass surface. Polymer coating was placed on the glass bars under the controlled conditions of temperature. The glass bars were placed in Methanol and water solution for 4 hours to prevent the destruction of extraction phase. In order to eliminate Pb(II), the adsorbent was stirred in Nitric acid (0.50 M) solution for 30 Min. Extraction parameters such as pH, volume of extraction solution, kind of elution solution, concentration of elution solution, volume of elution solution, time of extraction, time of desorption and extraction temperature were studied and optimized. The adsorption capacity of Pb(II) on IIP is 12.6 mg/g. The relative selectivity factor (?r) values of Pb(II)/Ni(II), Pb(II)/Cu(II), Pb(II)/Cr(II) and Pb(II)/Fe(II) are 3.78, 3.84, 0.88 and 3.20, respectively. The detection limit was found to be 0.024 mg/l with AAS and 0.106 µg/l with ICP. Pb(II) concentration factor of 100 was obtained with this method. The IIP has been successfully applied for the recovery of trace Pb(II) in real drinking, mineral and river water samples and those spiked with standards. Percent recovery of Pb(II) in water samples and the standard deviation of measurement with IIP were obtained (91.3 – 98.8) and ( 10) respectively.