In this research, ability of secondary electrospray ionization (SESI), as a nonradioactive ionization source in ion mobility spectrometry (IMS) was evaluated and compared with electrospray ionization (ESI). The effects of nonvolatile dopants including chloride, bromide and iodide dopants on sensitivity were investigated. In this work, SESI-IMS coupled with dispersive liquid liquid microextraction (DLLME) technique and used for determination of 2,4,6-trinitrotoluene (TNT). The effects of different parameters such as type and volume of extraction solvent, type and volume of disperser solvent, pH and the amount of salt were evaluated and optimized. Under the optimized conditions, calibration curve was plotted and the linear dynamic range of 0.4 to 150 µg.L -1 was obtained for analysis of TNT. The limit of detection was 1.0 µg.L -1 with intra and inter-day RSDs of 5 and 7%, respectively. finally, real samples of Defense Industry wastewater, river and drinking waters were analyzed and the obtained results reveal the capability of proposed method for TNT analysis.
