The liver is one of the largest and most complex organs of the body. The liver has many functions that are essential for life. Therefore, finding the applicable method of liver tissue manufacturing for tralantation, in cases where we are dealing with liver problems, is very important. Tissue engineering is the most widely used and successful method of tralantation and replacement different organs. The use of textile structures as scaffolds for tissue engineering already been suggested in several studies. This is because of the ability of these products to provide optimal spatial arrangement and the arrangement of the cells to maintain good nutritional condition of structural factors such as the pores of the-fiber-reinforced. In this study, in order to prepare Polycaprolacton nanofibers liver scaffold with high orientation distribution, a novel collector has been designed which is connected to a stepper motor and turn with a period of time to get to desired orientated nanofibers in the scaffold. The materials used to build as the base polymer of ano-fibers was ?- polycaprolactone with a molecular weight of ¯ (M_n) = 80000 obtained from Sigma - Aldrich, Germany. Dichloromethane solvent DMF was used as input. Dichloromethane solvent is good but a poor solvent for the polymer is DMF, but the electrolyte is very good and make the fibers delicate which produced by operation of the electro-spun. In this study, some wires on the rotating circular collector through diagonal direction were punched to control collection of the nanofibers on the collector. The wires were connected to the negative and the syringe containing polymer solution was connected to the positive pole of high voltage, to form nanofibers on the wires like spider web. Collecting the nanofibers have been controlled and helped to stretch and improve fiber fineness. The collector was based on the principle that form a circular plate collectors with wires on the diameter of circle collector and the collector was connected to a stepper motor which causes the collector rotates with a special speed. The purpose is producing a web like a spider web and fibers with high orientation distribution. Experiments were done by varying the distance between the wires together on the screen and change the speed of the collector plate. To determine the optimal conditions for production, difference speeds of designed collector and the angles of the wires on it were examined. The results of collector with angle of 30 ° and a rotational speed of 60 rpm had the optimal conditions and the highest distribution of orientation. The results also indicate that the ratio of rotational speed and angular position of the wires had wide effect on fiber orientation where the collector with the angle of wires 40° and the rotational speed is 90 rpm produces nanofibers more orientated than nanofibers on collector with angular of the wire 40° and rotational speed of 60 rpm. Nanofibers on the collector with angular of the wire 30° and rotational speed of 60 rpm were more orientated than nanofibers on collector with angular of the wire 30° and rotational speed 90 rpm. Therefore, to achieve greater distribution of orientation angle of wires and the rotational speed of the collector plate is related together. Key words : liver scaffold , polycaprolactone , nano-fiber