This work examines the dehydrogenation of 2-butanol over ruthenium-based cataly The effect of support type (ZnO/Al 2 O 3 ) and ruthenium loading on methyl ethyl ketone (MEK) yield were studied. The influence of structural and phase changes occurring in co-precipitated zinc-aluminum hydroxide system on performance of Ru-ZnO-Al 2 O 3 catalysts were studied. The operational factors such as reaction temperature, 2-butanol feed flow rate, calcinations temperature of the support, space velocity, and time on stream were studied. The highest MEK yields were obtained with a 6 wt. % ruthenium on ZnO-Al 2 O 3 -35 support. The optimum catalyst was used to measure the best working condition for dehydrogenation reaction at temperatures from 230 to 310 ?C. st.