Nanotechnology due to its wide applicability in many areas of science and technology is used in new products. In this field, the researchers tried to combine nanofiber with other nanomaterials to achieve new or improved features. Nanoparticles, are nanomaterials that due to their distinctive features and a variety of consumption are used in many cases. Nanofibers and nanoparticles combined together to produce results that actually make advantage of the performance and characteristics of both materials. In this study, the material are TiO2 and Polyacrylonitrile (PAN). PAN solution and TiO2 nanoparticles were combined and PAN/TiO2 nanofibers was produced. The aim of this work is study of wicking behavior in a nanofiber yarn. SEM images was taken and the fiber diameter was measured. With increasing concentrations of polymer solution and nanoparticles, nanofibers diameter increased. By increasing yarn twist, liquid height at equilibrium in the thread, fell. By the presence of nanoparticles in the fiber structure, the height of liquid rise in yarn increased, which indicates a reduction in the radius of the capillary space. By keeping the twist and nanoparticle in constant value and increasing in polymer solution concentration, the capillary rise furthered, but it was not noticeable and statistically significant. Ignoring the effect of gravity on liquid math, fluid behavior with Lucas–Washburn equation were compared. The radius of the capillary tubes were considered more accurate and after measuring viscosity and surface tension of the liquid r.cos? was calculated. By increasing the twist, in most cases r.cos? declined. The tensile strength of the yarns were measured and the results showed that with increasing concentration, strength also increased. Keywords: wicking phenomenon, nanofiber yarn, PAN, nanoparticle, twist, solution concentration