Conservation and sustainable use of genetic resources is essential to meet food security. Success in crop improvement generally depends on the magnitude of genetic variability in the germplasm. In order to study genetic variation for agronomic traits in a collection of Iranian and foreign safflower genotypes, an experiment was performed at the research farm of Isfahan University of Technology using a simple lattice design of 10×10 with three replications in 2011. In this experiment, 83 foreign genotypes along with 17 Iranian genotypes were evaluated. Agronomic and morphological traits including days to 50% flowering, days to maturity, plant height, number of branches per plant, head diameter, number of heads per plant, number of seeds per head, 1000 grain weight, grain yield per plant, seed yield per m 2 , biological yield, harvest index, seed oil content and oil yield were assessed. The result of analysis of variance showed that genotypes differed significantly for all of the studied traits. Foreign accessions showed better performance for number of heads per plant and had lower days to flowering, days to maturity and plant height indicating that foreign germplasm can be used in breeding programs to improve these traits.. High broad-sense heritability and low differenc were observed between genetic and phenotypic coefficients of variation for most of the traits. The highest broad- sense heritability was belonged to 1000 seed weight (95%) and the lowest one was estimated for number of branches per plant (68%). Genetic and phenotypic correlation coefficients showed that number of branches per plant, number of heads per plant, number of seeds per head and head diameter had positive and significant correlation with seed yield. The results of stepwise regression showed that number of heads per plant, number of seeds per head and 1000 grain weight explained 87 percent of seed yield variations . According to the results of path analysis, number of heads per plant had the most direct positive effect on seed yield; however, its effect was declined through indirect and negative effect of number of seeds per head and grain weight. Factor analysis recognized four factors which explained 82 percent of total variations. These factors were named as efficiency, physiological source, phenological factor and yield component of grain weight. Cluster analysis base on the agronomic and morphological traits grouped the genotypes into four clusters which comprised 59, 8, 5 and 28 genotypes, respectively. Iranian accessions was clearly separated from other genotypes and allocated in fourth group.. In conclusion, the results indicated that there was a broad genetic diversity among Iranian and foreign germplasm which can be exploited in breeding programs. Key Words: Agronomic traits, foreign genotypes, genetic diversity, safflower