High yield potential and grain quality of triticale derived from wheat parent and its resistance to stresses derived from rye parent. To evaluate morpho- physiological and grain quality traits of 18 triticale lines comprising 9 doubled haploid (DH) and 9 corresponding F6 lines, an experiment was conducted at research farm of College of Agriculture , Isfahan University of Technology located in Lavark- Najaf Abad in 2007. A randomized complete block design with 4 replications was used. Results of analysis of variances revealed significant differences among genotypes for most of studied traits with the exception of SDS sedimentation volume. The tested DH and F6 lines differed significantly for most of studied traits with the exception of the followings: number of spikes per area unit, grain weight per spike, SDS sedimentation volume, test weight, gliadine and length of flag leaf. The F6 line number 9 possessed the highest grain yield. Significant and positive correlations were observed between grain yield and with number of spikes per area unit, number of grain per spike, grain weight per spike, 1000 grain weight, biological yield, harvest index, length of flag leaf and flag leaf area. Among grain yield components, number of spike per area unit possessed the highest correlation with grain yield. The highest genetic coefficient of variation was observed for chlorophyll and the lowest one was for days to maturity. The highest and the lowest broad heritability estimates were 99.5? for plant height and 28.3? for SDS sedimentation volume, respectively. Also grain yield heritability was low. The results of stepwise regression showed that number of spike per area unit, grain weight per spike and spike length contributed in 98.6? of observed variation for grain yield. The results of stepwise regression showed that gliadin and glutenin- secalin could justify 52? of observed variation for grain protein. The results of factor analysis indicated 6 factors which explained 62.73? of total variation of data. The results of path analysis on the remaining characters in the regression model showed that grain weight per spike had the highest positive direct effect on grain yield. Effective grain filling rate among genotypes were 0.75 to 2.18 mg per day. The highest and the least effective grain filling rate were observed for DH line numbers 16 and 12. A negative and significant correlation was observed between effective grain filling rate and period. Overall, DH lines performed superior for agro- morphological traits and F6 lines were superior for physiological and grain quality related traits. It seems plausible to use grain weight per spike and number of spike per area unit as a selection criterion in breeding programs for producing high yield cultivars of