Drought stress as the most important abiotic stress plays an important role in the yield reduction of crop plants worldwide. For improvement of complex traits such as yield possessing low heritability, indirect selection is applied by using other traits viz morph-physiological traits. The study of physiological response of different cultivars to water stress could be beneficial in understanding of the mechanisms of drought resistance. Therefore this study was conducted to evaluate the effect of water stress on the morphological, physiological and quality of five triticale )X . Triticosecale Wittmack( genotype including one sensitive genotype and four resistance genotypes based on germination test, as well as one bread wheat and one durum wheat cultivars at Research Farm of Isfahan University of Technology. A randomized complete block design with three replication was used in each of two environment (i.e. irrigation after 70 and 130 mm evaporation from class A pan, respectively). Results of analysis of variance revealed significant differences among cultivars for most of studied traits under non stress condition with the exception of the followings: relative water content (RWC), initial water content (IWC), weight of flag leaf, gluten dry weight, protein percentage, protein content (TSP), gliadin and glutenin content. under moisture stress condition there were significant differences among cultivars for most of studied traits with the exception of chlorophyll a, RWC, and rate of water loss (RWL). The results of combined analysis showed that water stress significantly effected on most of the traits with the exception of IWC, awn length, weight of flag leaf, number of stomata, angle of flag leaf and gluten dry weight. The correlation coefficients revealed that traits related with leaf water content had negative effect and leaf area, number of stomata had positive effect on grain quality traits, under moisture stress. Only excised leaf water retention (ELWR) had significant and negative correlation with grain yield. In non stress condition width and area of flag leaf and angle of flag leaf had significant and negative correlation with grain yield. ELWR had significant and positive correlation with yield. The results of factor analysis under non stress condition indicated 7 factors explained 87.6% and 88.9% of total variation of yields under non stress and stress conditions, respectively. The results of stepwise regression showed that ELWR and awn dry weight contributed in 90% of observed variation for grain yield under non stress condition. Under stress condition rate of water loss contributed in 64% of observed variation for grain yield. The results of stepwise regression under non stress condition showed that ELWR and IWC contributed in 91% of observed variation for grain protein. Under stress conditions flag leaf area, leaf rolling and rate of water loss contributed in 99% of