Drought stress as a physical stress is one of the most important constrains of growth and production of crop plants worldwide. Attemps to develop new cereals with high yield potential and stability have been conducted and triticale is the only successful case. Triticale acquired superior yield potential and grain quality drived from wheat parent and the resistance to stress drived from rye parent. To evaluate physiological traits and grain quality of triticale lines (9 doubled haploid (DH) and 9 corresponding F7 lines) as well as two bread wheat cultivars (Roshan and Kavir), an experiment was conducted at research farm of College of Agriculture, Isfahan University of Technology located at Lavark- Najaf Abad in 2008. A randomized complete block design with 3 replications was used in each of two environmental conditions of irrigation after 70 and 130 mm evaporation from a analysis of variances revealed significant differences among genotypes for most of the studied traits with the exception of length and width of flag leaf and grain dry mater. Results of analysis of variances also revealed significant differences among F7 lines for most of studied traits with the exception of excised leaf water reduction (ELWR)‚ length (stress condition) and width of flag leaf. DH lines varied significantly for most of studied traits with the exception of ELWR‚ length and width of flag leaf‚ yield and grain dry matter. The results of combined analysis of variances showed that water stress significantly affected most of traits with the exception of some physiological traits and grain dry mater‚ nonfiber carbohydrates (NFC)‚ protein content (TSP)‚ test weight and gluten dry weight. grain yield Significantly and positively correlated with NFC, superoxide dismutase (SOD)‚ length of flag leaf and ELWR but the correlations with glutenin content was negative under normal condition. Under water stress conditions leaf water content‚ chlorophill a/b and NFC correlated with grain protein content negatively. Positive correlation was observed between leaf area‚ leaf angle‚ ELWR‚ lipid peroxidation (LP) and grain quality traits. under water stress conditions explained ascorbate peroxides (APX) was negative correlated with grain yield. The highest genetic variations under normal and Key words: Triticale‚ physiological traits‚ grain quality‚ doubled haploid lines‚ drought tolerance.