A general approach has been designed to synthesize mononuclear orthopalladated complexes containing bidentate ligands from the binuclear and mononuclear orthopalladated complex of triphenylphosphite. In this way, PdCl 2 was refluxed with PdCl 2 [P(OPh) 3 ] 2 in toluene to obtain the orthopalladated complex [Pd( m -Cl)P(OPh) 2 (OC 6 H 4 )] 2 ( 1a ) which was used as a precursor to react with bidentate ligands. dppe, d, dppf and in CH 2 Cl 2 at room to get mononuclear and binuclear complexes; [Pd( (?-dppe)cl(P(OPh) 2 (OC 6 H 4 )] 2 (1b) [Pd(d)cl(P(OPh) 2 (OC 6 H 4 )] (2b) [Pd( dppf)cl(P(OPh) 2 (OC 6 H 4 )] (3b) 4b)) Cl 2 [Pd ( (?-) (P(OPh) 2 (OC 6 H 4 )] 2 The complexes were fully characterized by using FT-IR spectroscopy, Elemental analysis, multinuclear NMR spectroscopy ( 1 H, 13 C and 31 P). In the second part of this project, the biological activity of the complexes by testing their ability to connect to the CT-DNA (calf thymus) Uv-Vis spectroscopy with the aid of fluorescence, DNA denaturation was evaluated. Also in this section, complex interactions with BSA, by means of fluorescence spectroscopy, UV-Vis array survey was conducted. UV absorption spectrum of protein in the absence and in the presence of various concentrations of behavior that had complexes reduction and displacement toward longer wavelengths represents the interaction of the complexes with the DNA and BSA also showed that changes in the intensity of absorption bands the interaction between BSA and BSA complexes changes in formulations and also change the polarity of the microenvironment surrounding the amino acids. By examining the complex interactions between different drugs with the BSA in the presence of the marker sites, site links to each BSA molecule complex was proposed. The final section examines the effects of synthetic anti-bacterial compounds.