Graphene oxide is extensively recognized as an effective material in a biomedical application and especially for drug delivery application. The aim of this study was to develop a novel drug carrier via facile coating of nipam-itaconic acide dual pH-temperature responsive copolymer on the surface of Graphene oxide for the efficient loading and reducing side effect of anticancer agent. For this reason, at the first step copolymerization of itaconic acide and nipam occurred in the presence of initiator and cross linker. Secondly, nipam/itaconic acide was grafted on the surface of different content of graphene oxide. The oxidize groups of graphene oxide nano sheets enables them to attach to the amide groups of graphene oxide with hydrogen bond interactions. The lower critical solution temperature of the GO(PNIPAAm-Co-ITA) have been investigated by Differential scanning calorimetry. The result shows that the lower critical solution temperature of the PNIPAAm increase with the presence of graphene oxide. In order to evaluate the pH sensitivity of nanoparticles, the equilibrium swelling ratio of nano core shell particles under conditions of different pH was evaluated. The result show that these particles were able to absorb large amount of water in all media. Subsequently, GO(PNIPAAm-Co-ITA) nanoparticles were characterized by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction. TEM images show the core shell structure of GO(PNIPAAm-Co-ITA) with smaller size than 100nm.
