These days, nanofibrous materials are gaining great application in biomedical fields, including drug release device, tissue regeneration matrices and tissue engineering. For tissue regeneration, composite nanofibers are favored due to their mechanical and biological properties. Moreover, use of biopolymer as a composite matrix allows easy fabrication and provides a level of mechanical felexibility. A bio-scaffold ca be broadly defined a a structure used to repair and regenerate of damaged tissue through the cell migration into the defect site. The scaffold can be made from natural or synthetic materials. Many types of polymers (natural and synthetic ) are widely used in research associated with human retinal eye disease. The main advantages of scaffolds made from composites or synthetic polymers are : extensive structure, various properties in comparison with natural polymers, capability of choosing undegradable polymer or degradable polymer under specific condition. Poly (glycerol sebacate ) (PGS) is a degradable polyester which is synthesized through a polycondensation reaction of glycerol and sebacic acid under high temperature and vacuum. Its degradation products of hydrolysis are nontoxic and undugous natural metabolites. These properties make PGS a good candidate for cornea and retina tissues repair. In this thesis, the PGS prepolymer was synthesized by polycondensation of equimolar glycerol and sebacic acid at 120 °C under nitrogen atmosphere for 24 h. Then synthesized prepolymer was dried at 50 o C under vacuum (200 mbar). Therefore, a viscous and traarent prepolymer was obtained which can be dissolved in different solvents. Thereafter, polymer characterization was carried out using FTIR, X-ray, and DSC. The results showed that the synthesized polymer has the properties similar to PGS that was synthesized in previously published literature. In the next step, prepolymer solutions were prepared using different ratios of polycaprolactone in various solvents such as tetrahydrofurane (THF), ethanol (EtOH), chloroform (ClF), dimethylformamide (DMF), and dichloromethane (DCM). All solutions were electrospun under different conditions of spinning distance, applied electric voltage, and flow rate. SEM and optical microscope images of electrospun fibers showed that spinning distance of 15 cm, electric voltage of 10 kV, and flow rate of 0.3 ml/min are the optimal conditions to obtain free-bead fibers. Then, PGS/PCL (1:1) were dissolved in DCM to prepare 30 %(wt/v) solution. Electrospinning of this solution was performed under the mentioned optimal condition and led to fibers without any bead. In the next step, mechanical and degradation properties of nanofiber webs were measured. Degradation analysis showed that after 28 days, the weight loss of the samples immersed in phosphate buffer solution at 37 °C and pH = 7.4, was only 40%. The reason of this low rate of degradation of PGS/PCL nanofibers is higher crystallinity of PCL component . The mechanical measurements indicated that tensile modulus of nanofiber web is 0.1 MPa, approximately equal to that of the retina tissue. Key Word Condensation polymerization, scaffold, nanofiber, poly(glycerol sebacate ), electerospinning, retina, tissue engineering