In this project, synthesis of ZSM-5 zeolite membrane and permeation of hydrogen, carbon monoxide, methane and nitrogen gases from the produced membrane was studied. Among the zeolite membranes we choose ZSM-5 because of its high selectivity and also its average permeability for gases mixture. For synthesis ZSM-5 zeolite membrane first nano particles of MFI zeolite (silicalite-1) in particle sizes of 25 and 80 nm were synthesized. In nano particle synthesis, two-stage varying temperature synthesis was used and to increasing synthesis efficiency and decreasing synthesis time and template consumption, the microwave assisted hydrothermal method was utilized. The microwave method and mole composition of nano particles synthesis solution which is used in this project is entirely unique. The produced nano particles were analysized by TEM, SEM, EDX, XRD and FT-IR. In seeding of ?- alumina supports the Silicalite-1 nano particles was used and ZSM-5 zeolite membrane was synthesized by secondary growth method. Zeolite membrane synthesis was performed in two ways: 1- template-free synthesis method and 2- synthesis of thin layer membrane by using a little amount of template. The structure of produced membranes was studied by EDX, XRD and SEM analysis and the performance by gas separation set up. The thickness of thin layer membrane was about 2 ?m. Because of the important effect of some parameters such as temperature and pressure on membrane separation, the effect of those parameters on membrane function (the permeability and ideal selectivity) was studied in gas separation experiments with varying the levels. The results of gas separation show that by increasing temperature the permeability of pure gases decrease and the ideal selectivity of membrane increases. The increase of pressure causes insignificant decrease on permeability of pure gas and also slight increase in membrane selectivity. Experiments show that working in a temperature of 298K and pressure of 120 KPa, we can reach to suitable amount of permeability and selectivity for hydrogen. The gained permeability in this circumstance with the best thin layer membrane of 2?m thickness was 2.6 mol/m 2 s pa for hydrogen and 6.1 mol/m 2 s pa for carbon monoxide. Ideal selectivity of H 2 /CO in this situation is 4.59.