The fungal like organism, Albugo candida (pers) Roussel from family Albuginaceae, order Albuginales, Saskatoon university. After transformation by agroinfilteration in Arabidopsis and tobacco non of the samples caused morphologic signs in these plant. In canola both of the constructs showed the disease signs chlorosis. Then eight construct were sequenced. Two sequences which showed the disease signs, were compared with other pathogenic proteins containing Nep1 domain by Blastx and Blastp which pointed the adaptation. By signalp software, the existences of marker peptide were verified in these two sequences that causes the secretory property in protein. Therefore, the sequences which were adapted with two pathogenic sequences by doing Blast, were choose for phylogenetic relationship comparison by MEGA4 software, then the phylogenetic tree were constructed. From two sequences regarding A. candida, one is located in some group with Sclerotinia sclerotiorum and another one is located in very close branch to this group. These result showed harmony with the blast results in which these two fungi had most similarity with fungal like A. candida. Then the canola leaf samples were used for 2d-electrophresis and the staining results showed very little amount in protein extraction, so that it was impossible to observe the differences between the treatment samples and control samples. According to what was observed, it can be concluded that was maybe in the stage of protein extraction, the protocol was not efficient and there is a need to optimize it for canola plant. Key words : Transient expression , Pathogen effectors molecules , Agroinfilteration , Albugo candida
